ABSTRACT
AIM To study the chemical constituents from the fruits of Evodia rutaecarpa (Juss.) Benth.and their biological activities.METHODS The ethyl acetate fraction of 80％ ethanol extract from E.rutaecarpa was isolated and purified by silica column and recrystallization,then the structures of obtained compounds were identified by physicochemical properties and spectral data.The antitumor and antifungal activities were determined by MTT and mycelium growth method,respectively.RESULTS Ten compounds were isolated and identified as rutaecarpine (1),evodiamine (2),1-methyl-2-undecyl-4 (1H)-quinolone (3),1-methyl-2-tridecyl-4 (1H)-quinolone (4),wuchuyuamide Ⅰ (5),wuchuyuamide Ⅲ (6),wuchuyuamide Ⅳ (7),β-sitosterol (8),β-daucosterol (9),dehydroevodiamine (10).Compounds 1,2,10 had certain inhibitory effects on MDA-MB-231,LoVo,A2780 and HeLa with the IC50values of 0.65-29.45 μmol/L.Compound 1 had a certain inhibitory effect on Botrytis cinerea,Glomerella cingulata and Rhizoctonia solani with the inhibitory rates of 18.12％-49.57％.CONCLUSION Compounds 3-7 are isolated from this plant for the first time,compounds 1,2,10 have strong biological activities.